Effects of Reactive Oxygen Species on Development of Porcine Parthenogenetic Embryos in Vitro

(December 2016 - Vol 99 No 4,  pp. 401-407)

Author(s): Junhe Hu, Junmin Wang, Chenzhong Jin, Zhongbin Yang, Zhi Zeng, Juan Wu, Xianglin Liu, Jian Zhao


Reactive oxygen species (ROS) is one of the most important factors that affect early embryos during their in vitro development. This study determined the effect of different oxygen tensions on the early developmental ability of porcine embryo and the quality of porcine parthenotes in vitro. Two methods were chosen to culture parthenogenetic embryos in vitro (A, 20% oxygen for 1-6 d for the control group and B, 5% oxygen for 1-6 d). ROS level in embryos significantly decreased in 5% oxygen culture compared with that in the 20% oxygen culture (P<0.05). The frequency of blastocyst formation and the total number of nuclei in each blastocyst in treatments with low oxygen were significantly higher than the values in the control group (54.06% vs. 35.47%; P<0.05). The relative transcript abundance of P66Shc and BAX gene products in porcine embryos in the control group was significantly higher than that in the group subjected to 5% oxygen for 1-6 d (P<0.05). However, the relative expression of Bcl-XL gene in porcine embryos in the control group A was significantly lower than that in the group subjected to 5% oxygen for 1-6 d (P<0.05). The number of apoptotic cells in each blastocyst was also significantly lower in embryos cultured under 5% oxygen compared with 20% oxygen conditions. These results suggest that low oxygen culture can improve the development and quality of porcine embryos by improving Bcl-XL expression and inhibiting P66Shc and BAX expression to prevent oxidative damage-induced apoptosis in the process of porcine embryo development in vitro.


Keyword(s): apoptosis, embryo, parthenogenetic development, porcine, reactive oxygen species


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