Analysis of the APX Gene Expressed in Soybean Infected by Heterodera glycines and Coated with Biocontrol Bacteria Sneb545

(December 2016 - Vol 99 No 4,  pp. 365-369)

Author(s): Peng Xiang, Feng Zhu, Jingsheng Chen, Hongpeng Li, Wencheng Lu, Baohua Li, Lijie Chen, Yuxi Duan

 

The response of ascorbate peroxidase (APX) gene to nematode (Heterodera glycines) infection was studied in roots of soybean cultivars ? the susceptible Liaodou 15 and Liaodou 15 coated with bio- control bacteria Sneb545. The expression profile of APX in coated Liaodou 15 infected with H. glycines, which generates oxidative stress, was analyzed. Real time PCR analysis was carried out with root extracts of infected plants 4, 7, 11, and 14 d after nematode inoculation and using uninfected roots a control. PCR analysis revealed that APX mRNA levels increased from 1.08- to 1.46-fold after exposure to H. glycines from 4 to 7 d, and decreased to 0.87- and 0.92-fold from 11 to 14 d compared with the non- inoculated control. These results suggest that the APX gene was induced by H. glycines at an early stage in soybean roots and may play an important role in removing oxidative damage.

 

Keyword(s): ascorbate peroxidase, Heterodera glycines, real time PCR, soybean, soybean cyst nematode

 

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December 2016 - Vol 99 No 4

 

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