Effects of Klebsiella pneumoniae Strain SnebYK on Atrazine Metabolism in Soybean Leaves

(December 2016 - Vol 99 No 4,  pp. 348-354)

Author(s): Le Chen, Dan Liu, Xiaofeng Zhu, Yuanyuan Wang, Yuxi Duan


Atrazine is a widely used herbicide in agriculture. Despite its agronomic importance in weed control, it is toxic to dicotyledonous crop plants such as soybean; residual activity of atrazine can result in damage to this crop. In a previous study, evidence suggested that the bacterial Klebsiella pneumoniae strain SnebYK induced resistance to atrazine in soybean. To understand the mechanism behind these observations, the effects of SnebYK on the activity and expression of the enzymes involved in herbicide metabolism were analyzed. SnebYK applied to seeds stimulated the activation of glutathione S-transferase (GST), peroxidase (POD), glutathione reductase (GR), and glutathione peroxidase (GPX) after treatment with atrazine. In addition, SnebYK enhanced the expression of P450, GR, GPX and MRP which encode cytochrome P450 monooxygenase, glutathione reductase, glutathione peroxidase and multi-drug resistance-associated proteins, respectively. The messenger RNA (mRNA) levels of the glutathione S-transferase genes (GST22, GST14 and GST18) were also increased after atrazine treatment, which was mediated by SnebYK. These data suggest that SnebYK may be suitable for wider applications to enhance the tolerance of soybean to atrazine exposure.


Keyword(s): atrazine, enzyme, induced resistance, Klebsiella pneumoniae, soybean, qRT-PCR


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December 2016 - Vol 99 No 4


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